Journal: Frontiers in Pharmacology

Article Title: Barnesiella intestinihominis improves gut microbiota disruption and intestinal barrier integrity in mice with impaired glucose regulation

doi: 10.3389/fphar.2025.1635579

Figure Lengend Snippet: Barnesiella intestinihominis improved glucose tolerance in IGR mice with high-fat diet. (A) A schematic diagram of the study design. (B) Weight changes in IGR mice induced by a high-fat diet during the study period: normal control group (NC group) n = 8, IGR group n = 16. (C) IPGTT experimental curve before B. intestinihominis treatment, (D) is the area under the curve of (C) . (E) IPITT experimental curve before B. intestinihominis treatment, (F) is the area under the curve of (E) . (G) IPGTT experimental curve after B. intestinihominis treatment, (H) is the area under the curve of (G) . (I) IPITT experimental curve after B. intestinihominis treatment, (J) is the area under the curve of (I) , n = 8, (K) insulin resistance index of mice after B. intestinihominis treatment (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Barnesiella intestinihominis (B. intestinihominis , strain: DSM 21032) was obtained from the German Microbial Strain Conservation Center DSMZ and cultured in minced meat broth (Mingzhou, Zhejiang, China, K0747) under anaerobic conditions (10% CO 2 , 10% H 2 , 80% N 2 ).

Techniques: Control

Journal: Frontiers in Pharmacology

Article Title: Barnesiella intestinihominis improves gut microbiota disruption and intestinal barrier integrity in mice with impaired glucose regulation

doi: 10.3389/fphar.2025.1635579

Figure Lengend Snippet: Barnesiella intestinihominis improved intestinal inflammation in IGR mice. (A–C) Inflammation factors in mouse colon tissue. (D) Map of LPS levels in serum of mice. (E) HE staining of mouse colon tissue (Small frame magnification: ×50; Large frame magnification: ×200). n = 6, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Barnesiella intestinihominis (B. intestinihominis , strain: DSM 21032) was obtained from the German Microbial Strain Conservation Center DSMZ and cultured in minced meat broth (Mingzhou, Zhejiang, China, K0747) under anaerobic conditions (10% CO 2 , 10% H 2 , 80% N 2 ).

Techniques: Staining

Journal: Frontiers in Pharmacology

Article Title: Barnesiella intestinihominis improves gut microbiota disruption and intestinal barrier integrity in mice with impaired glucose regulation

doi: 10.3389/fphar.2025.1635579

Figure Lengend Snippet: Barnesiella intestinihominis restores GM dysbiosis in IGR mice with high-fat diet. (A–C) The changes of Sob index, Chao index and Ace indexes (D) NMDS map after B. intestinihominis intervention. (E) PLS-DA map after B. intestinihominis intervention. (F) GMHI chart of IGR mice with high-fat diet. (G) Three sets of MDI maps. (H) Pie chart of microbiota composition in three groups of mice. (I) Analysis diagram of species composition difference among the three groups of mice. (J) Correlation analysis heat map. n = 6, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Barnesiella intestinihominis (B. intestinihominis , strain: DSM 21032) was obtained from the German Microbial Strain Conservation Center DSMZ and cultured in minced meat broth (Mingzhou, Zhejiang, China, K0747) under anaerobic conditions (10% CO 2 , 10% H 2 , 80% N 2 ).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Barnesiella intestinihominis improves gut microbiota disruption and intestinal barrier integrity in mice with impaired glucose regulation

doi: 10.3389/fphar.2025.1635579

Figure Lengend Snippet: Barnesiella intestinihominis intervention increased expression of ZO-1 and occludin in IGR mice. (A) Western Blot of ZO-1 and occludin expression in colon tissue. (B,C) Quantitative Western Blot of ZO-1 and occludin in colon tissue. (D,E) Relative quantitative map of ZO-1 and occludin genes in colon tissue by RT-qPCR. n = 6, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Barnesiella intestinihominis (B. intestinihominis , strain: DSM 21032) was obtained from the German Microbial Strain Conservation Center DSMZ and cultured in minced meat broth (Mingzhou, Zhejiang, China, K0747) under anaerobic conditions (10% CO 2 , 10% H 2 , 80% N 2 ).

Techniques: Expressing, Western Blot, Quantitative RT-PCR

Journal: Frontiers in Pharmacology

Article Title: Barnesiella intestinihominis improves gut microbiota disruption and intestinal barrier integrity in mice with impaired glucose regulation

doi: 10.3389/fphar.2025.1635579

Figure Lengend Snippet: Barnesiella intestinihominis fermentation broth increases viability of Caco-2 cell and ZO-1 expression. increased after heat-inactivated broth treatment. (A) Effect of bacterial solution on cell viability. (B) Effect of co-culture of DSS with bacterial broth on cell viability. (C,D) Relative quantitative RT-qPCR profiles of intercellular tight junction protein ZO-1 and occludin gene. (E) Western blot of intercellular tight junction proteins ZO-1 and occludin expression. (F,G) Quantitative Western blot of intercellular tight junction proteins ZO-1 and occludin. (H,I) ZO-1, occludin expression level was estimated by Immunofluorescence assay (* p < 0.05).

Article Snippet: Barnesiella intestinihominis (B. intestinihominis , strain: DSM 21032) was obtained from the German Microbial Strain Conservation Center DSMZ and cultured in minced meat broth (Mingzhou, Zhejiang, China, K0747) under anaerobic conditions (10% CO 2 , 10% H 2 , 80% N 2 ).

Techniques: Expressing, Co-Culture Assay, Quantitative RT-PCR, Western Blot, Immunofluorescence

Journal: Frontiers in Pharmacology

Article Title: Barnesiella intestinihominis improves gut microbiota disruption and intestinal barrier integrity in mice with impaired glucose regulation

doi: 10.3389/fphar.2025.1635579

Figure Lengend Snippet: Barnesiella intestinihominis has no significant effect on Caco-2 cell monolayer barrier permeability. (A) Schematic diagram of the establishment of the Caco-2 monolayer cell barrier model. (B) TEER of Caco-2 cells with time. (C) Caco-2 cell morphology observed on day 21. Scale bar = 100 μm. (D,E) Changes in TEER and ALP activities before and after intervention (* p < 0.05).

Article Snippet: Barnesiella intestinihominis (B. intestinihominis , strain: DSM 21032) was obtained from the German Microbial Strain Conservation Center DSMZ and cultured in minced meat broth (Mingzhou, Zhejiang, China, K0747) under anaerobic conditions (10% CO 2 , 10% H 2 , 80% N 2 ).

Techniques: Permeability

Journal: Molecular Systems Biology

Article Title: Diet-driven differential response of Akkermansia muciniphila modulates pathogen susceptibility

doi: 10.1038/s44320-024-00036-7

Figure Lengend Snippet:

Article Snippet: Barnesiella intestinihominis : YIT11860 , DSMZ , Cat # DSM 21032.

Techniques: Blocking Assay, Sequencing, Modification, Saline, Bacteria, SYBR Green Assay, Staining, Software, Microscopy, Spectrophotometry, Enzyme-linked Immunosorbent Assay, Flow Cytometry